Detailed view for EmuJ_000735200

Basic information

TDR Targets ID: 957895
Echinococcus multilocularis, cleavage stimulation factor subunit 2
Source Database / ID:  GeneDB

pI: 5.7786 | Length (AA): 402 | MW (Da): 42948 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00076   RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
PF14304   Transcription termination and cleavage factor C-terminal
PF14327   Hinge domain of cleavage stimulation factor subunit 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0031124   mRNA 3'-end processing  
GO:0003676   nucleic acid binding  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
12 114 1p1t (A) 10 110 60.00 0 1 1.05732 -1.46
20 177 2mjn (A) 97 257 20.00 0.00000000048 1 0.609035 0.22
112 193 2l9b (A) 146 227 22.00 0 0.91 0.51808 -0.78
112 193 4eba (G) 112 193 21.00 0 0.94 0.43608 -0.3
355 399 2j8p (A) 529 573 47.00 0.00000064 0.77 0.73694 -1.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128452)

Species Accession Gene Product
Arabidopsis thaliana AT1G71800   cleavage stimulating factor 64
Babesia bovis BBOV_I001450   RNA recognition motif domain containing protein
Brugia malayi Bm1_06205   cleavage stimulation factor
Candida albicans CaO19.12013   similar to cleavage and polyadenylation factor CF I component involved in pre-mRNA 3'-end processing
Candida albicans CaO19.4538   similar to cleavage and polyadenylation factor CF I component involved in pre-mRNA 3'-end processing
Caenorhabditis elegans CELE_F56A8.6   Protein CPF-2
Cryptosporidium hominis Chro.10152   cleavage stimulation factor
Cryptosporidium parvum cgd1_1310   cleavage stimulation factor subunit 2 that has a CSF1 like RRM domain
Drosophila melanogaster Dmel_CG7697   Cleavage stimulation factor 64 kilodalton subunit
Echinococcus granulosus EgrG_000735200   cleavage stimulation factor subunit 2
Echinococcus multilocularis EmuJ_000735200   cleavage stimulation factor subunit 2
Homo sapiens ENSG00000101811   cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Homo sapiens ENSG00000177613   cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
Loa Loa (eye worm) LOAG_12614   cleavage stimulation factor
Mus musculus ENSMUSG00000031256   cleavage stimulation factor, 3' pre-RNA subunit 2
Mus musculus ENSMUSG00000053536   cleavage stimulation factor, 3' pre-RNA subunit 2, tau
Neospora caninum NCLIV_015280   mRNA processing protein, putative
Oryza sativa 4349923   Os11g0176100
Plasmodium berghei PBANKA_0833800   CSTF domain-containing protein, putative
Plasmodium falciparum PF3D7_0933000   CSTF domain-containing protein, putative
Plasmodium knowlesi PKNH_0731700   CSTF domain-containing protein, putative
Plasmodium vivax PVX_087055   mRNA processing protein, putative
Plasmodium yoelii PY06426   hypothetical protein
Plasmodium yoelii PY02603   synthetic antigen of P.falciparum, putative
Saccharomyces cerevisiae YGL044C   Rna15p
Schistosoma japonicum Sjp_0302420   similar to Cleavage stimulation factor 64 kDa subunit, tau variant, putative
Schistosoma mansoni Smp_079730   rna recognition motif containing protein
Schmidtea mediterranea mk4.026879.00  
Schmidtea mediterranea mk4.002801.04   Cleavage stimulation factor 64 kilodalton subunit
Toxoplasma gondii TGME49_283740   RNA recognition motif-containing protein

Essentiality

EmuJ_000735200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_F56A8.6 Caenorhabditis elegans embryonic lethal wormbase
YGL044C Saccharomyces cerevisiae inviable yeastgenome
TGME49_283740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EmuJ_000735200 (Echinococcus multilocularis), cleavage stimulation factor subunit 2
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